Overall percent agreement=97.2% (493/505); 95% CI (95.4, 98.4)1. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2. Clipboard, Search History, and several other advanced features are temporarily unavailable. 2019 Jul 29;15(7):e1007914. 2019 Jul 17;93(15):e00289-19. In cases of suspected early disease, a repeat serum specimen should be collected 14 to 21 days later and submitted for testing.The presence of IgG-class antibodies to either HSV type 1 or 2 does not differentiate between remote infection or acute disease.HSV serology cannot distinguish genital from nongenital infections.The predictive value of positive or negative results depends on the prevalence of disease and the pretest likelihood of HSV-1 and HSV-2.False-positive results may occur. This site needs JavaScript to work properly.
Two of these 9 specimens were positive by WB; 2 of these 9 specimens were equivocal by WB. The HerpeSelect IgG assays distinguish between HSV-1 and HSV-2 on the basis of differences in the patient’s immune response to HSV glycoprotein G (gG). However, recent studies have demonstrated that the same proteins also play a key role in initiating the early innate immune response to HSV.

These assays are based on purified recombinant glycoprotein G-1 (HSV-1) or G-2 (HSV-2) antigens.

It is suggested that, following gD binding to one of its entry receptors, membrane fusion is mediated by gB and the heterodimer gH/gL. Brown ZA, Selke S, Zeh J, et al: The acquisition of herpes simplex virus during pregnancy. This review summarises the known structure and function of each of these essential viral envelope glycoproteins, and explores what is known about their close cooperation with each other in mediating cellular membrane fusion. Because IgG antibodies to HSV persist for life, serologic assays can detect infection even in the absence of lesions. 2019 Jul 30;20(15):3715. doi: 10.3390/ijms20153715.Ye X, Gui X, Freed DC, Ku Z, Li L, Chen Y, Xiong W, Fan X, Su H, He X, Rustandi RR, Loughney JW, Ma N, Espeseth AS, Liu J, Zhu H, Wang D, Zhang N, Fu TM, An Z.PLoS Pathog. Four viral surface glycoproteins (gB, gD, gH and gL) are required. 2019 Apr 2;16(1):9. doi: 10.1186/s12977-019-0470-5. HSV-1 encodes at least 85 genes and 12 of these genes code for glycoproteins (1–6).These glycoproteins (gB, gC, gD, gE, gG, gH, gI, gJ, gK, gL, gM, and gN) are the major inducers and targets of humoral and cell-mediated immune responses following infection (4, 7–10).We have constructed recombinant baculoviruses … Envelope glycoprotein that forms spikes at the surface of virion envelope. HSV type 2 primarily causes urogenital infections and is found almost exclusively in adults. Ashley RL, Wu L, Pickering JW, et al: Premarket evaluation of a commercial glycoprotein G-based enzyme immunoassay for herpes simplex virus type-specific antibodies.
Binnicker MJ, Jespersen DJ, Harring JA: Evaluation of three multiplex flow immunoassays to enzyme immunoassay for the detection and differentiation of IgG-class antibodies to herpes simplex virus types 1 and 2. All Rights Reserved. However, in instances of subclinical or unrecognized HSV infection, serologic testing for IgG-class antibodies to type-specific HSV glycoprotein G (gG) may be useful. J Clin Microbiol 1998;36:294-2953. N Engl J Med 1997;337:509-5154. Determining whether a patient has been previously exposed to herpes simplex virus (HSV) types 1 and 2Distinguishing between infection caused by HSV types 1 and 2, especially in patients with subclinical or unrecognized HSV infectionHerpes simplex virus (HSV) types 1 and 2 are members of the Infections with HSV types 1 and 2 can differ significantly in their clinical manifestations and severity. The herpes simplex viruses are both similar and different and are often misunderstood. Most HSV serology assays, however, are not type-specific. Wiley